Do you've got a protocol or proposed antibodies to complete ICC staining on my absolutely differentiated ALI cultures?

Blend Carefully ahead of transferring cells to some conical tube made up of ten mL of pre-warmed wash medium. Be aware: Clean the pipette idea and cryovial with the clean medium at the least three instances to assist strengthen Restoration.

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Mucus is usually washed from the surface area of the cells at the time weekly starting up at week 3, once the cells are already cultured in PneumaCult™-ALI Medium.

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Likely again to the plate, rinse Every nicely with 1 mL of FACS buffer and transfer the amount on the fifteen mL tube. Observe: Retain cell suspension on ice after transfer to the tube till prepared to run FACS.

Differentiation into adipocytes, osteoblasts, or chondrocytes with suitable MesenCult™ differentiation systems

Of course, you’ll discover the measures to perform an ICC staining on the epithelial cells cultured with the ALI On this 지방이식 protocol. Here's a listing of antibodies that may be used for the characterization of airway cultures:

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Normally, HSAECs cultured in PneumaCult™-ALI-S Medium will type a fully differentiated cuboidal epithelium soon after four to 5 weeks of society. Some donor variability may very well be predicted.

A minimum amount reaction quantity of 50 µL is needed for steady efficiency with the CellPore™ Transfection System.

This can be an stop-position protocol intended to dissociate PSC-derived kidney organoids into one-cell suspension and fixation for downstream stream cytometry Investigation.

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